PhD student, University of Nottingham United Kingdom University of Nottingham Bulwe, England, United Kingdom
Objectives: Identify the bioactive components in Cyperus esculentus Investigate reproductive effect of Cyperus esculentus on cadmium chloride-induced toxicity
Methods: Liquid chromatography mass-spec and gas chromatography mass -spec was employed in bioactive analysis.
Results: Cyperus esculentus, also called tiger nuts is cultivated for its edible tubers, referred to earth almonds. Liquid chromatography mass-spec and gas chromatography mass -spec was employed in bioactive analysis. GC-MS shows the presence of 11-octadecenoic acid, methyl ester (23.28%), hexadecanoic acid methyl ester (16.84%), docecanoic acid, methyl ester (15.81%), 2,3-dihydroxypropyl ester (8.90%), methyl tetradecanoate (8.86%), oleic acid (8.28%), octadecanoic acid, methyl ester (4.85%), octanoic acid, methyl ester (3.75%), undecanoic acid, methyl ester (3.58%), docosanoic acid, methyl ester (1.82%), octadecanoic acid, 2-hydroxy-1,3-propanediyl ester (1.8%), docosanoic acid, methyl ester (1.05%), bicyclo[3.1.1]hept-2-ene,2,6,6-trimethyl (0.58%), heneicosanoic acid, methyl ester (0.41%), hexadecanoic acid,2-hydroxy-1-(hydroxyl methyl) ethyl ester (0.20%). LC-Ms indicates malonamic acid with 104.224, 6-0Acetylaustroinulin with 365.025 amount. Sperm motility significantly impaired in CdCl2-treated group relative to control. Sperm motility decreased drastically following treatment with C. esculentus, improved testosterone level, percentage of morphologically abnormal sperm increased significantly in the experimental control. Most of the compounds identified in the plant have mainly antimicrobial properties. However C. esculentus could help in curbing infection caused by microbes, reduce the impact of cadmium chloride toxicity in reproductive indices while the histological observation of the ovaries in experimental control shows slight follicular necrosis compare to treated group which shows reversal effects.
Conclusions: C. esculentus could help in curbing infection caused by microbes, reduce the impact of cadmium chloride toxicity in reproductive indices and improve reproductive health.
Funding Sources: PTDF (Petroleum Trust Development Fund)
