(P04-064-24) Hyperglycemic Rats Exposed to West Mexico Blackberries Improved Metabolic Parameters Similar to Healthy Rats

Objectives: The objective of this work was to evaluate West Mexico blackberries potential to ameliorate markers related to type 2 diabetes in vivo.

Methods: Wistar rats (n=40, 50% female and 50% male) were employed in two experimental phases. In the first one (8 weeks); 32 were exposed to ad libitum 30% sucrose solution (SS30), rodent standard diet, and an intraperitoneal (IP) injection of 15 mg/streptozotocin/kg bw. 8 rats were exposed to purified water, standard diet, and an IP injection of 1 mL of distilled water.

For the second phase (8 weeks), groups (n=8) were formed, continuing with SS30 or purified water and the following interventions: control healthy group (HC); negative control exposed to SS30 (NC). Three groups with SS30 + blackberry gel (BG). B-x: BG dose of 2.5 mg C3G (cyanidin 3-glucoside) eq/kg bw/day; B-2x: BG dose of 5 mg C3G eq/kg bw/day; B-3x: BG dose of 7.5 mg C3G eq/kg bw/day. Every week, body weight (BW), abdominal circumference (AC), craniocaudal length (CCL), and body mass index (BMI) were assessed. Serum glucose, lipid profile, insulin, leptin, atherogenic risk index (ARI), HOMA2-IR, and adiposity index were analyzed. Ethics protocol number (CUCBA/CEIC/CE/001/2020), University of Guadalajara.

Results: Rats exposed to SS30 developed higher AC (14.6±1.4 vs 12.5±0.8 cm, p < 0.05) and BMI (0.66 ±0.08 vs 0.58±0.07), compared to HC. After the exposure to BG, B-x group showed lower BW (262±76 g), AC (12.5±1), and BMI (0.62±0.1), and B-2x lower AI. Among BG groups, the glucose (83±18.4 mg/dL), total cholesterol (60±10 mg/dL), ARI (0.26±0.08, p < 0.05), insulin (2.26±0.65 μUI/mL), HOMA2-IR (0.37±0.03) and leptin (174.7±74.98 pg/mL) levels were lower in B-3x. The NC group developed higher levels of all the parameters evaluated. The evaluated parameters in B-3x were similar compared to HC group.

Conclusions: Results suggest that blackberry could act as an adjuvant in the treatment of noncommunicable diseases and represent a useful product for developing economically sustainable functional foods.

Funding Sources: Virgen-Carrillo was supported by a scholarship (733116) from the Consejo Nacional de Humanidades, Ciencias y Tecnologías, Conahcyt, México.