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Dietary Bioactive Components (Poster Session)

(P04-051-24) The Bioavailability, Anti-Inflammatory Effects, and Metabolomic Signature of Ginger 6-Shogaol in Lipopolysaccharide-Induced Inflammation in Mice

Sunday, June 30, 2024
12:45 PM – 1:45 PM CT
Location: Poster Board 124

    Presenting Author(s)

  • FO

    Femi E. Omoniyi

    Graduate Student
    North Carolina A&T State University
    Kannapolis, North Carolina, United States

    • Co-Author(s)

  • SZ

    Shuwei Zhang, PhD

    North Carolina A&T State University
    Kannapolis, North Carolina, United States

  • Pei-Sheng Lee, PhD (she/her/hers)

    Post-Doctoral Research Associate
    North Carolina A&T State University
    Kannapolis, North Carolina, United States

  • SS

    Shengmin Sang, PhD

    Distinguished Professor
    North Carolina A&T State University
    Kannapolis, North Carolina, United States

Disclosure(s):
Femi E. Omoniyi: No relevant financial relationship(s) with ineligible companies to disclose.
Shengmin Sang, PhD: No relevant financial relationship(s) with ineligible companies to disclose.


Objectives: Ginger is widely used for its anti-inflammatory properties. However, there remains a gap in our understanding of the bioactivity of specific functional compounds present in ginger and how their modulation can translate into health benefits. In this study, the specific objectives were to investigate the bioavailability and potential anti-inflammatory effect of 6-shogaol (6S), a major bioactive compound in aged ginger, against lipopolysaccharide (LPS)-induced acute inflammation in mice. Furthermore, we explored the biological signature of 6S in combating inflammation using liquid chromatography-mass spectrophotometry (LC-MS) based untargeted metabolomics.

Methods: Male CD-1 mice were randomly divided into six groups including positive and negative control groups and 6S treatment groups (5, 10, 20 and 40 mg/kg respectively) (n = 12/group). 6S was administered via oral gavage for 15 consecutive days. On the final day, mice were injected with LPS (2.5 mg/kg) to induce inflammatory response, followed by euthanasia six hours later. Inflammatory biomarkers were quantified using ELISA kits while the bioavailability and metabolomic investigations were carried out using HPLC-ECD and LC-MS.

Results: Preliminary results showed that 6S actively attenuates the inflammatory effects of LPS as observed in the TNF-α and IL-6 quantification in both plasma and liver tissues of mice. A dose response effect was observed in the bioavailability of 6S and its metabolites in plasma and tissues of treated mice. Furthermore, the untargeted metabolomic analysis identified 6S and its metabolites as the exposure biomarkers of 6s, and endogenous metabolites that are related to inflammation as the functional biomarkers of 6S.

Conclusions: Overall, this study not only demonstrated the potential anti-inflammatory effects of 6S in murine plasma and tissues but also identified both the exposure and functional biomarkers of 6S. These findings offer mechanistic insights into the anti-inflammatory effects of ginger.

Funding Sources: This work was partially supported by funding from NIH grant R01HL144852.