(P18-020-24) Phenolic Compounds Identified in Extracts From Unique Mexican Common Beans Present Anti-aging Potential: In-Silico and In-Vitro

Objectives: Evaluate in silico and in vitro the anti-aging potential of compounds present in crude and phenolic-enriched extracts from two unique bean varieties (Phaseolus vulgaris L.) through the adenosine monophosphate-activated protein kinase (AMPK) and Sirtuin1 (Sirt1) pathway.

Methods: Black bean (BB) and pinto bean (PB) crude extracts (CE) were obtained through conventional leaching with 50% ethanol (0.3% formic acid). Afterward, phenolic-enriched extract (PHE) from each variety was obtained using XAD7 resin. Phenolic compounds were identified and quantified by HPLC. Molecular docking was performed in the active sites of AMPK and Sirt1 with identified phenolic compounds. Sirt1 and NFκB p65 protein expression levels were measured through western blot in the Hs27 fibroblast cell line treated with particulate matter composed of polycyclic aromatic hydrocarbons and heavy metals and co-treated with extracts and pure phenolic compounds.

Results: Four anthocyanins and eight phenolic compounds were quantified. The most abundant non-colored phenolic compounds were syringic acid with a concentration of 10.8 mg/g dry extract (DE) in PHE-PB, gallic acid with 8.2 mg/g DE, quercetin-3-D-galactoside with 4.4 mg/g DE, chlorogenic acid with 3.6 mg/g DE and caffeic acid with 3.3 mg/g DE in PHE-BB. Delphinidin-3-glucoside was the most potent compound binding with AMPK (-9.0 kcal/mol) with interaction in the active site's relevant residues Arg 83 and Asp38. Petunidin-3-glucoside interacted with Sirt1 (-6.6 kcal/mol), presenting a hydrogen bond with Pro 212 relevant residue in the active site. Rutin increased the expression of Sirt1 in the Hs27 cell line by 30%, and NFκB p65 subunit expression showed no statistical difference among the pure phenolic treatments (p > 0.05).

Conclusions: Delphinidin and petunidin glucosides presented high affinity to AMPK and Sirt1 active sites. Rutin increased the expression of Sirt1 in Hs27 fibroblasts exposed to particulate matter. Phenolic compounds from native common beans could be used to develop functional foods and cosmeceutical products.

Funding Sources: The author was supported by a scholarship from CONACYT-México, number 901000 and FORDECYT-México grant number 292474.