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Food Science and Nutrition (Poster Session)

(P09-019-24) Compound Dietary Fibers From Whole Grains and Soybeans Ameliorate Obesity-Associated Insulin Resistance via Activating AMPK/GLUT4 Pathway

Sunday, June 30, 2024
12:45 PM – 1:45 PM CT
Location: Poster Board 297

    Presenting Author(s)

  • Mingxia Jiang, Doctor (she/her/hers)

    Director of Clinical Nutrition Department
    Dushu Lake Hospital Affiliated to Soochow University
    Suzhou, Jiangsu, China (People's Republic)

    • Co-Author(s)

  • CL

    Chengjie Ling

    Dushu Lake Hospital Affliated to Soochow University, China (People's Republic)

  • YC

    Yin Cheng

    Henan Vocational College of Nursing, China (People's Republic)

  • CZ

    Chengkai Zhai

    Southeast University

Disclosure(s):
Mingxia Jiang, Doctor: No relevant financial relationship(s) with ineligible companies to disclose.


Objectives: Health benefits associated with the consumption of whole grains have been confirmed by numerous studies. Dietary fiber (DF), an important component of whole grains, is widely accepted to be responsible for these health benefits. Whole-grain dietary fibers (DFs) is demonstrated to reduce risks of insulin resistance (IR) and diabetes. However, most of the current studies had focused on DFs from a single source of plant foods. Evidence regarding the effects of compound DFs was limited. The present study aimed to explore the impact of compound DFs on IR associated with obesity and compare it with the effects of DFs from single whole grains or soybeans.

Methods: After one week of adaptive feeding, 60 SD rats weighed 180-220g were randomly divided into 6 groups: the normal diet group (N), the high-fat diet group (H), the wheat DF group (W), the kaoliang DF group (K), the soybean DF group (S), and the compound DF group (C). Purified feeds based on the formula of AIN-93MI731was used for the normal diet (ND, 16.2% from fat). This intervention lasted for 12 weeks. Every 4 weeks, blood glucose and insulin levels were analyzed. After intervention, the rats were euthanized by CO2 inhalation, and the liver, muscle and fat tissues were collected and weighed for further analysis.

Results: Compared with the HFD, compound DFs supplementation attenuated fasting blood glucose (FBG), insulin level and HOMA-IR via activating AMPK signaling and upregulating GLUT4 expression at mRNA and protein levels. It was also accompanied by improvements of serological index, and includes blood lipids, inflammatory cytokines, adipokines and antioxidant activity. Body weight gain was alleviated after the compound DF intervention. Besides, the compound DF improved liver steatosis induced by a HFD and increased liver antioxidant capacity. The results of comparing the compound and single DFs showed that the compound DF supplementation resulted in lower FBG and HOMA-IR compared to kaoliang DF.

Conclusions: The compound DF could improve abnormal glucose metabolism induced by HFD and showed potential advantages over single DF.

Funding Sources: This work was supported by the National Natural Science Foundation of China (No. 81372986).