Skip to main content
NUTRITION 2024 Main banner
Icon Legend
This session is not in your schedule.
This session is in your schedule. Click again to remove it.
Back

Precision Nutrition/Nutrient-Gene (Poster Session)

(P29-017-24) Anti-inflammatory Effects of Lycium barbarum Polysaccharides and/or Bifidobacterium longum in Rats With DSS-Induced Ulcerative Colitis

Monday, July 1, 2024
11:45 AM – 12:45 PM CT
Location: Poster Board 132

    Presenting Author(s)

  • Pei-Ching Lee

    Graduate student
    Taipei Medical University, School of Nutrition and Health Sciences
    Taipei, Taipei, Taiwan (Republic of China)

    • Co-Author(s)

  • YL

    Yu Zhi Lian, PhD

    doctoral student
    School of Nutrition and Health Sciences, Taipei Medical University
    Taipei, Taipei, Taiwan (Republic of China)

  • TN

    Tomonori Nochi

    Tohoku University, Miyagi, Japan

  • CC

    Chun-Chao Chang

    Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, 252 Wu-Hsing Street, Taipei, Taiwan

  • CL

    Chiou-Feng Lin

    Taipei Medical University, Taipei, Taiwan (Republic of China)

  • CH

    Chi-Chang Huang

    National Taiwan Sport University, Taoyuan, Taiwan (Republic of China)

  • Jane C.-J Chao, PhD

    Professor and Dean
    Taipei Medical University
    Taipei, Taipei, Taiwan (Republic of China)

Disclosure(s):
Pei-Ching Lee: No relevant financial relationship(s) with ineligible companies to disclose.
Jane C.-J Chao, PhD: No relevant financial relationship(s) with ineligible companies to disclose.


Objectives: This study investigated the protective effects of Lycium barbarum polysaccharides (LBP) and/or Bifidobacterium longum OLP-01 (OLP-01) on colonic inflammation in rats with dextran sulfate sodium (DSS)-induced ulcerative colitis (UC).

Methods: Male Sprague-Dawley rats aged 7-week-old were divided into 5 groups: normal (N), DSS-induced UC (U), UC and treated with 100 mg LBP/kg bw (L), UC and treated with 2 × 109 CFU OLP-01/kg bw (B), and UC and treated with both 50 mg LBP/kg bw and 1×109 CFU OLP-01/kg bw (M) groups. Treatments of LBP and OLP-01 were administered orally through gavage for 39 days. From day 29 to day 35, rats were orally administered 5% DSS in drinking water continuously for 7 days to induce UC. The colon was collected for analysis on day 39. Histological analysis for colonic damage was determined by hematoxylin and eosin stain. Colonic pro-inflammatory interleukin 6 (IL-6) levels and cyclooxygenase-2 (COX-2) protein were measured respectively by enzyme-linked immunosorbent assay and western blot.

Results: Histological observation showed ulceration of the epithelial layer, loss of normal mucosal architecture, and full-thickness inflammation of the colon wall accompanied by a significant increase in histological disease scores in the DSS-treated group. Rats supplemented with LBP and/or OLP-01 had less colonic damage with significantly lower histological disease scores compared to the DSS-treated group. The DSS-treated group significantly increased colonic IL-6 levels, while all treatment groups significantly decreased colonic IL-6 levels. Colonic COX-2 protein involved in inflammation was significantly increased in the rats with colitis, and was significantly decreased in all the treatment groups compared to that in the DSS-treated group.

Conclusions: LBP and/or OLP-01 alleviate colon damage with decreased histological disease scores, and inhibit inflammation via reducing IL-6 secretion and COX-2 protein expression in DSS-induced ulcerative colitis rats.

Funding Sources: This research was funded by the National Science and Technology Council, Taiwan.