(P18-029-24) Effects of Akkermansia muciniphila in Mouse Models of Metabolic and GI Disorders: A Meta-Analysis of Animal Studies

Objectives: The significance of Akkermansia muciniphila (A. muciniphila; a bacterial member of the human gut microbiome) is rising, emphasizing the need for exploring its potential in preventive strategies for various diseases. A. muciniphila and its cellular components, including extracellular vesicles and outer membrane proteins, have garnered recognition for their capacity to enhance intestinal homeostasis and metabolic health. We herein investigated the impact of A. muciniphila and its components on gastrointestinal (GI) and metabolic disorders using a meta-analysis of mouse model studies.

Methods: Eligible studies were identified through targeted searches on PubMed, Web of Science, Science Direct, and Embase until 30^th December 2023, totaling 39 studies.

Results: A. muciniphila and its derivatives (alive or non-alive) exhibited positive effects on systemic and gut inflammation, liver enzyme levels, glycemic response, and lipid profiles. The intervention increased the expression of tight-junction proteins in the gut, improving gut permeability in mouse models of GI and metabolic disorders. Regarding body weight, A. muciniphila and its derivatives prevented weight loss in animals with GI disorders while reducing body weight in high-fat diet-treated animals and mice with metabolic disorder. Sub-group analysis indicated that the use of live bacterium had a stronger effect on most analyzed biomarkers. Gut microbiome analysis using live A. muciniphila demonstrated a substantial difference in A. muciniphila levels between groups, although microbiome diversity did not significantly differ between the control and A muciniphila-treated groups. This suggests that A. muciniphila may impact metabolic responses through mechanisms beyond altering gut microbiome composition.

Conclusions: In conclusion, A. muciniphila and its components could be promising interventions to ameliorate gut inflammation and metabolic disturbances.

Funding Sources: This research was supported by the US Department of Agriculture (USDA-AFRI, GRANT12444832) and the Florida Department of Health, James and Esther King Biomedical Research Program (9JK01).