(P14-017-24) Immunomodulatory Effects of Oral Probiotics and Trametes versicolor Extracellular Polysaccharopeptides in Bowel-Resection Rats on Total Parenteral Nutrition

Objectives: Massive bowel resection and prolonged total parenteral nutrition (TPN) can induce immune dysfunction. Our study aimed to explore the impact of oral supplementation with probiotics, Trametes versicolor extracellular polysaccharopeptides (TV-ePSP), or their combination on immune responses in TPN-supported bowel-resection rats.

Methods: Male SD rats underwent bowel resection, from 10 cm beyond the ligament of Treitz to 5 cm proximal colon, and jugular vein catheterization. One day post-surgery, rats received deionized water (SBS group), probiotics (LB group, 10⁹ CFU of Lactobacillus acidphilus and 10⁹ CFU of Bifidobacterium bifidum), TV-ePSP (TVP group, 1 g/kg), or probiotics and TV-ePSP (COM group) daily for 6 days. Healthy, orally fed rats were served as references (R group).

Results: Results revealed that the SBS group had significantly reduced body weight, plasma interleukine-6 levels, and phagocytosis activity in peripheral blood leukocytes (PBL). Conversely, white blood counts (WBC), spleen weights, and granulocytes in PBL were elevated compared to the R group. The TVP group exhibited increased WBC, the LB group showed decreased spleen weight, and the COM group displayed elevated plasma IL-6 levels, B cells in PBL and splenocytes, and T-helper splenocytes, with reduced T-helper cells in PBL compared to the SBS group. Under lipopolysaccharide stimulation, splenocyte proliferation significantly increased in the SBS group, TNF-alpha and IL-6 secretions increased in the LB group, and TNF-alpha, interferon-gamma, IL-4, and IL-10 secretions increased in the COM group.

Conclusions: In conclusion, the combination of probiotics and TV-ePSP shows promise in enhancing immune function of splenocytes in bowel-resection rats on TPN.

Funding Sources: Ministry of Science and Technology of Taiwan