(P14-002-24) Antioxidant and Anti-inflammatory Properties of Aqueous Extract and Digested Samples of Lupins (Lupinus albus) in Different In Vitro Cell Models

Sunday, June 30, 2024

11:45 AM – 12:45 PM CT

Location: Poster Board 471

Presenting Author(s)

Donato Angelino, PhD (he/him/his)

Associate Professor
University of Teramo, Functional Food and Metabolic Stress Prevention Laboratory, Department of Bioscience and Technology for Food, Agriculture and Environment
Teramo, Abruzzi, Italy

Co-Author(s)

VD

Veronica D'Antonio

University of Teramo, Functional Food and Metabolic Stress Prevention Laboratory, Department of Bioscience and Technology for Food, Agriculture and Environment
Teramo, Italy
CB

Chiara Bravo trippetta

University of Teramo, Functional Food and Metabolic Stress Prevention Laboratory, Department of Bioscience and Technology for Food, Agriculture and Environment
Teramo, Italy
MR

Marina Ramal-Sanchez

University of Teramo, Functional Food and Metabolic Stress Prevention Laboratory, Department of Bioscience and Technology for Food, Agriculture and Environment
Teramo, Italy
MS

Mauro Serafini

University of Teramo, Functional Food and Metabolic Stress Prevention Laboratory, Department of Bioscience and Technology for Food, Agriculture and Environment
Teramo, Italy

Disclosure(s):

Donato Angelino, PhD: No relevant financial relationship(s) with ineligible companies to disclose.

Objectives: There is a growing interest for the health effects due to the consumption Lupinus species, as L.albus. They are source of vegetable proteins and soluble fiber, and rich in bioactive compounds. Thus, the lupin consumption may have a role in the modulation of oxidative and inflammatory markers. The present work aims at evaluating the antioxidant and anti-inflammatory properties in specific in vitro and ex-vivo cell models, by considering both the lupin aqueous extracts and the digesta.

Methods: Pickled lupin seeds were used for the preparation of i) aqueous extracts, after hexane defatting step; ii) digesta, following INFOGEST Protocol simulating gastrointestinal human digestion. Antioxidant properties were evaluated by measuring the reactive oxygen species (ROS) scavenging activity of lupine aqueous extracts (0.4 to 8 g d.m./L) incubated for 40 min with NCM460 intestinal cell lines, chemically stressed with 2,2'-Azo-bis-amidinopropane (ABAP).

Anti-inflammatory properties of lupin digesta (0.1 to 1 g d.m./L) were evaluated i) in CaCo-2 intestinal cells, modulation of CXCL-10 and IL-8 cytokines after 24h incubation, in the presence or absence of lipopolysaccharide (LPS) for 24h and 48h; ii) in ex-vivo cellular model with polymorphonuclear (PMNs) leukocytes and monocytes isolated from the blood of healthy subjects, modulation of TNF-α and IL-6 after 24h incubation and 24 h LPS stimulation.

Results: Lupin extracts incubated at 0.4 e 8 g d.m./L, but not 0.8 and 1.6 g d.m./L, significantly reduced ABAP-induced ROS production compared to ABAP-treated control NCM460 intestinal cells.

Lupin digesta 0.1, 0.25, 0.5, 1 g d.m./L significantly reduced, in CaCo-2 cells, the production of IL-8 after 48h and CXCL-10 after 24 and 48h, compared to the only LPS stimulated control cells.

Lupin digesta 0.1, 0.25, 0.5, 1 g d.m./L significantly inhibited after 24h the production of TNF-α, but not IL-6, induced by LPS in blood PMNs compared to only LPS controls.

Conclusions: Lupin extracts were able to modulate redox status of intestinal cells, while digesta showed an anti-inflammatory activity in intestinal cells and human PMNs. These findings support the development of human intervention trial to confirm the in vitro results.

Funding Sources: The research was founded by a grant of the Italian Ministry of the Economic Development.