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Dietary Bioactive Components (Poster Session)

(P04-065-24) Anti-colon Cancer Effect of Apio Tuber Extract

Sunday, June 30, 2024
12:45 PM – 1:45 PM CT
Location: Poster Board 138

    Presenting Author(s)

  • YW

    Yu-Yun Wang, Master

    Graduate assistant
    University of Arkansas

    • Co-Author(s)

  • OA

    Oluwatobi Amusat, MSc

    Graduate Student
    University of Arkansas
    Fayetteville, Arkansas, United States

  • AA

    Alok Arun

    Inter American University of Puerto Rico
    Barranquitas, Puerto Rico, United States

  • SL

    Sun-Ok Lee, PhD

    Associate Professor
    University of Arkansas
    Fayetteville, Arkansas, United States

Disclosure(s):
Yu-Yun Wang, Master: No relevant financial relationship(s) with ineligible companies to disclose.
Oluwatobi Amusat, MSc: No relevant financial relationship(s) with ineligible companies to disclose.


Objectives: Apio (Arracacia xanthorrhiza) is a starchy, flavrous, and nutritious root crop commercially popular in South America. The highly-demanded edible part of Apio is its corm. However, its byproduct, Apio tuber, is prone to being discarded and wasted. The objectives of the study are to measure the total phenolic content (TPC) and antioxidant activity and to evaluate anti-colon cancer effect of Apio white and yellow tuber to reduce waste by enhancing their value.

Methods: The Apio white tuber (AWT) and yellow tuber (AYT) samples used in this study were donated by Dr. Arun of the American University of Puerto Rico. The TPC of the sample extract was analyzed by the Folin-Ciocalto method. The free radical scavenging activity was determined by the 2,2- diphenyl-1-picrylhydrazyl (DPPH) method. Caco-2 cells were pretreated with 100-500 mg/mL of Apio tuber extracts. Cells were then incubated with or without lipopolysaccharide (LPS, 100 ng/mL) for nitric oxide (NO) production. Cell viability and NO production were measured using the MTS assay and Griess reagent kit, respectively. The results were expressed as mean ± standard deviation (mean ± SD). Statistical analysis was performed using JMP Pro Version 16 with significance at P < 0.05 level.

Results: The TPC in AWT and AYT were 2.04 ± 0.27, and 1.28 ± 0.11 mg GAE/g, respectively. The DPPH in AWT and AYT were 11.91 ± 2.32, and 9.81 ± 2.67 µM TE/g, respectively. AWT had significantly higher TPC and scavenging activity than AYT. For cell viability, 300 - 500 µg/mL of AWT significantly reduced cell viability in a dose-dependent manner. Both AWT and AYT (200 - 500 µg/ml) significantly decreased the LPS-induced production of nitric oxide.

Conclusions: The results showed that the Apio white tuber is a good source of phenolics that exert significant antioxidant activity and anti-colon cancer effects, suggesting that Apio tuber may be used as a functional food.

Funding Sources: No specific grant was funded for this research.