(P04-058-24) Red Kidney Beans Remediate the Hepatic Energy Metabolic Modulations Induced by the Diets Containing Saturated Fats As the Main Lipid Source

Objectives: High saturated fat (HSF) have been linked to impaired pathways involved in central carbohydrate metabolism, such as glycolysis, tricarboxylic acid cycle, and glutaminolysis. These detrimental effects can lead to multiple disorders. However, dry edible beans (DEBs) have been reported to promote multiple health benefits and may play a significant role in improving energy imbalances caused by HSF diet. Yet, a critical gap in knowledge exists on the impact of DEBs consumption on energy modulation. Thus, the objective of this study was to assess the ability of red kidney beans to remodulate the hepatic energy pathways and to understand the possible mechanisms behind the potential beneficial effects. Our hypothesis is that RKB will alleviate the damaging impact of HSF on hepatic energy homeostasis.

Methods: To test our hypothesis, Syrian hamsters were randomly grouped into five groups (n=11) and fed with one of five experimental diets: a normal fat diet (NF), a diet with saturated fats as the main lipid source (HSF), HSF with 5% of the whole bean, HSF with 4.5% dehulled bean and HSF with 0.5% hull of bean for 4 weeks. Different diets were used to provide a foundation to identify probable agents responsible for potential benefits by thoroughly characterizing the agents in RKB fractions.

Results: The results showed that HSF impaired the ability of the cell to generate energy from glucose by significantly inhibiting glycolysis. Subsequently, amino acid catabolism was upregulated in HSF model as a possible anaplerosis source to sustain the TCA, thereby producing glucose through gluconeogenesis. Despite the RKB supplementation enhanced the metabolic rate via glycolysis, amino acids metabolism, and inhibited gluconeogenesis. As such, supplementation resulted in remediating the effects of HSF in central carbon metabolism by sustaining glycolytic and the TCA cycle pathways in the livers regardless of HSF intake.

Conclusions: These results are expected to promote the developing healthier bean market classes that specifically target energy imbalances caused by HSF diets.

Funding Sources: USDA, UNL, Egyptian Government