(P15-017-24) Adipose Tissue-Derived Extracellular Vesicles as Agents of Metabolic Regulation and Potential Biomarkers of Insulin Resistance

Objectives: To analyze the metabolic and predictive role of Extracellular Vesicles of Adipose Tissue in the early stages of obesity.

Methods: As the first stage, a diet-induced obesity model was conducted for 10 weeks. In the second stage, the proteome contained in AT-derived EVs was analyzed to determine a molecular fingerprint that differentiates the obese/IR group.

Results: The group with obesity presented higher values compared to the control group, both in the Triacylglyceride Glucose Index (TyG) and in the Triacylglyceride/HDL-c ratio, both employed to estimate IR (9.2; 0.13 vs. 8.3; 0.27, 1.4; 0.2 vs. 0.8; 0.1 (p < 0.05)), respectively. Regarding EVs, their size was associated with TyG (r=0.63; p< 0.001), suggesting that the larger the EV size, the higher the IR. Finally, by bioinformatic comparison with different search engines, we found potential biomarkers of proteins involved in metabolic processes of the insulin signaling pathway and regulation of triacylglyceride synthesis in adipocytes.

Conclusions: These data suggest that both the biophysical properties, together with the protein content in EVs, could be used to identify early IR in people who consume high-fat diets, and who tend to increase their waist circumference as a consequence of weight gain or have obesity. With the above, it is expected to validate these plasma-derived EV proteins in people with obesity.

Funding Sources: Consejo Nacional de Humanidades Ciencias y Tecnologías