(P09-035-24) Daily Mango and Probiotic Consumption Modulates Metabolite Profile In Adolescents: A Randomized, Double-Blinded, Placebo-Controlled Study

Objectives: To assess if daily mango consumption on the presence of has an effect in gallotannin metabolites in plasma and urine, which are correlated to changes in inflammatory biomarkers and cognitive function.

Methods: For this study, healthy adolescents (ages 13-17 years) were recruited, including 17 females and 19 males with a BMI ranging 18-40 kg/m². Participants were divided into two groups (J and S), one consumed 250 g of mango puree treated with tannase (5 U/g) and a probiotic blend, the other consumed 250 g of mango puree without tannase and a placebo capsule, they consumed the treatment daily, for 60 days. On days 0, 30 and 60, plasma and urine were collected from participants before and 2 hours after treatment. Samples were analyzed in LC-MS and the metabolic profile was determined. Currently, the different mango treatments remain blinded.

Results: Pyrogallol sulfate concentration was found to be significantly higher in plasma (0.31 to 9.87 mg/L, p=0.01) and urine (91.91 to 263.78 mg/L, p< 0.0001) for group T, it increased when comparing day 0 and 60. This could be attributed to gallotannins hydrolysis, decarboxylation by microbiota, and liver sulfation. When comparing the metabolites in urine between genders, 3-hydroxybenaldehyde (0.20 in males and 0.49 mg/L in females, p=0.026) and benzoic acid (0.19 in males and 0.54 mg/L in females, p=0.049) were found to be significantly higher in females compared to males on the last day of the study.

Conclusions: Daily mango consumption significantly increased the concentration of pyrogallol sulfate in urine and plasma for the participants that had treatment J. Differences between male and female subjects were also detected, the concentration of 3-hydroxybenaldehyde and benzoic acid was higher in female subjects. These results suggest that daily mango consumption helps increase gallotannin metabolites which are associated with improvements regarding inflammatory biomarkers and cognitive function.

Funding Sources: Partially funded by the USDA-AMS-National Mango Board, Orlando, Florida