(P27-037-24) Effects of Lactiplantibacillus plantarum dfa1 and High-Fiber Dessert Diet on Akkermansia Abundance and Metabolic Health in Mice

Objectives: This study assessed Lactiplantibacillus plantarum dfa1 (Lp dfa1) on the gut microbiome, particularly on Akkermansia, and its metabolic health impact in mice. It focused on diets with varied macronutrient ratios, including Prachuap Khiri Khan’s Pineapple Cheese Cake Biscuit for its fiber content.

Methods: Twenty-five mice were divided into five groups for a 12-week study:
- Group 1 Control (RD) with a C:P:F ratio of 56:13:31.
- Group 2 High Glucose Diet (HGD) with a C:P:F of 60:25:15.
- Group 3 HGD+Lp received HGD with Lp dfa1 supplementation at 1x10^9 CFU daily.
- Group 4 High Dessert Diet (HDD) included the high-fiber biscuit, adjusting RD to a C:P:F of 70:9:21.
- Group 5 HDD+Lp combined the high-fiber diet with Lp dfa1 daily.

Measurements included Akkermansia abundance via 16S rRNA sequencing and metabolic health indicators: body weight, fat thickness, plasma cholesterol, glucose, IL-6, and liver profiles.

Results: HDD+Lp significantly increased Akkermansia to 8.245% (±4.802), surpassing HDD’s non-detectable levels (p=0.007) and HGD’s 0.381% (p=0.396). HGD+Lp slightly increased Akkermansia to 1.352% (±1.247), not significantly different from HGD (p=0.518). Additionally, HDD+Lp mice demonstrated notable metabolic improvements: body weight gain was reduced by 6%, subcutaneous fat thickness by 32%, plasma cholesterol by 17%, glucose levels by 20%, and IL-6 by 27%. Liver health also improved, with a 22% reduction in cholesterol and 28% in triglycerides, highlighting the dietary intervention’s comprehensive benefits.

Conclusions: Lp dfa1 with a high-fiber dessert diet significantly boosted Akkermansia and improved metabolic health in mice, demonstrating the synergy between probiotics and dietary fiber in enhancing gut and metabolic health.

Funding Sources: This research is funded by the National Research Council of Thailand.