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Nutritional Epidemiology (Poster Session)

(P13-028-24) Discovery of Banana Intake Biomarkers Among Chinese Population

Sunday, June 30, 2024
11:45 AM – 12:45 PM CT
Location: Poster Board 417

    Presenting Author(s)

  • GZ

    Geng Zong

    Shanghai Institute of Nutrition and Health

    • Co-Author(s)

  • Han Han, PhD Candidate

    Student
    Shanghai Institute of Nutrition and Health
    Boston, Massachusetts, United States

    • Co-Author(s)

  • WS

    Wanning Shang

    Shanghai Institute of Nutrition and Health, China (People's Republic)

  • ZX

    Zilin Xiao

    Shanghai Institute of Nutrition and Health, China (People's Republic)

  • PL

    Peiyu Li

    Shanghai Institute of Nutrition and Health, China (People's Republic)

Disclosure(s):
Han Han, PhD Candidate: No relevant financial relationship(s) with ineligible companies to disclose.


Objectives: Food intake biomarkers (FIBs) would improve the estimation of banana intake in epidemiological studies and help investigate the associations between banana consumption and long-term health. We aimed to discover novel banana FIBs among the Chinese population.

Methods: A randomized crossover dietary intervention, containing 3 consecutive stages (single-dosage [SD], multiple-dosage [MD], and follow-up period [FU]), was performed on 18 healthy volunteers. Volunteers underwent 2 dietary interventions: 1) control food, and 2) banana (240/480g in total as in SD/MD period, respectively) + control food. Urine samples were collected and analyzed with untargeted metabolomics based on LC-MS. A multi-tiered screening strategy matched to the study design was developed and applied to identify potential biomarkers.

Results: We identified 63 metabolites related to banana consumption, of which 9 metabolites, hydroxytyrosol, phenylcarbinol, dopamine, 5-hydroxyindoleacetic acid, methyl 3,4,5-trimethoxycinnamate, 6-methoxyeugenol, 2-isopropylmalic acid, 4-hydroxy-3-methoxybenzenemethanol, and salsolinol, were selected as candidate FIBs. Most of these metabolites showed a kinetic excretion pattern peaking within 0-6h after banana intake and their urinary level returned to baseline at 24h. Though hydroxytyrosol and dopamine remained discriminative for banana intake at 24h, only dopamine showed a significant difference compared to baseline during the FU period. The results suggested potential dose-response relationships between hydroxytyrosol/methyl 3,4,5-trimethoxycinnamate and banana intakes.

Conclusions: We identified 9 metabolites as potential banana FIBs. The metabolic profiles might limit their potential to reflect habitual banana intake individually. Panels of candidate biomarkers should be considered and require further validation in independent population studies.

Funding Sources: National Natural Science Foundation of China: 82373576