(P09-012-24) The Effects Of 100% Orange Juice on Skin Parameters and Oxidative Stress and Inflammation Biomarkers in Women in a Randomized Controlled Trial

Objectives: This study aimed to evaluate the impact of 100% orange juice on the skin parameters and biomarkers of oxidative stress and inflammation in healthy women aged 40-65 using a randomized, single-blinded, placebo-controlled, and cross-over design.

Methods: The research was approved by IRB and registered on https://clinicaltrials.gov (NCT04861623). Twenty healthy women aged 40-65 with Fitzpatrick skin types 2 and 3 were recruited. Following a 14-day run-in period, participants were assigned to consume either 12 oz orange juice or placebo juice (Sunny D orange drink) for ten weeks, separated by a 28-day wash-out period. Skin parameters were measured at baseline, end of week 5, and end of week 10. Biomarkers for oxidative stress and inflammation in the blood were assessed using ELISA. Effects of treatment, time, and their interactions were analyzed using two-way ANOVA and mixed-effects models with Tukey’s tests. Changes in skin parameters from baseline were compared with paired t-tests.

Results: Orange juice consumption attenuated the increase of melanin index on the face after five weeks (p=0.025) and ten weeks (p=0.068) compared to placebo. Orange juice positively affected wrinkles on the inner forearm but not on the face. However, there was no effect on UVB-induced erythema, transepidermal water loss, erythema index, color, firmness, or elasticity compared to placebo juice consumption on the face or inner forearm. Advanced glycation end products, IL-6, tumor necrosis factor, high sensitivity C-reactive protein, and matrix metalloproteinase-9 in plasma, superoxide dismutase, and glutathione peroxidase activities in erythrocytes remained comparable after orange juice or placebo juice intake. Time is the most significant factor affecting skin parameters, followed by treatment and time-treatment interaction. Ten weeks of orange juice significantly increased the hydration levels on the face (57.6 ± 3.3 vs 64.5 ± 2.9, p=0.03 for time, p=0.11 for treatment), as well as on the inner forearm (39.5 ± 2.5 vs 46.5 ± 3.0, p < 0.01 for time, p=0.97 for treatment) compared to the baseline.

Conclusions: This study revealed that consuming 100% orange juice for ten weeks had little impact on skin parameters and did not affect women's oxidative stress and inflammation biomarkers.

Funding Sources: Florida Department of Citrus